Teko ea protheine ea electrophoresis

Teko ea protheine ea electrophoresis (UPEP) e sebelisetsoa ho hakanya hore na liprotheine tse itseng li ka morong joang.

Mohlala oa moroto o hloekileng o hlokahale. Mokhoa oa ho tšoasa o hloekileng o sebelisetsoa ho thibela likokoana-hloko tse tsoang botoneng kapa botšehaling hore li se ke tsa kena sampoleng ea moroto. Ho bokella moroto oa hau, mofani oa tlhokomelo ea bophelo a ka u fa sesebelisoa se khethehileng sa ho ts'oasa se hloekileng se nang le tharollo ea ho hloekisa le ho hlakola ha nyopa. Latela litaelo hantle.

Kamora hore u fane ka sampole ea moroto, e romelloa laboratoring. Ha a le moo, setsebi sa laboratori se tla beha sampole ea moroto pampiring e khethehileng ebe e sebelisa motlakase. Liprotheine lia sisinyeha ebe li theha lihlopha tse bonahalang. Tsena li senola boholo ba protheine ka 'ngoe.

Mofani oa hau a ka u joetsa hore u khaotse ho noa meriana e itseng e ka sitisang tlhahlobo. Meriana e ka amang liphetho tsa liteko e kenyelletsa:

• Chlorpromazine
• Corticosteroids
• Isoniazid
• Neomycin
• Phenacemide
• Li-salicylates
• Sulfonamides
• Tolbutamide

Se ke oa emisa ho noa moriana leha o sa bua le mofani oa hau pele.

Teko ena e kenyelletsa feela ho ntša metsi ka mokhoa o tloaelehileng. Ha ho na bothata.

Ka tloaelo ha ho na protheine, kapa ke protheine e nyane feela ka morong. Protheine e phahameng ka mokhoa o sa tloaelehang ka har'a moroto e ka ba sesupo sa mathata a mangata a fapaneng.

UPEP e kanna ea khothaletsoa ho thusa ho tseba sesosa sa protheine morong. Kapa e kanna ea etsoa joalo ka teko ea ho hlahloba ho lekanya mefuta e fapaneng ea mefuta e fapaneng ea liprotheine morong. UPEP e lemoha mefuta e 'meli ea protheine: albin le li-globulin.

Ha ho na li-globulin tse ngata tse fumanehang morong. Albumine ea moroto e ka tlase ho 5 mg / dL.

Mekhahlelo e tloaelehileng ea boleng e ka fapana hanyane hara lilaboratori tse fapaneng. Li-lab tse ling li sebelisa litekanyo tse fapaneng kapa li leka liteko tse fapaneng. Bua le ngaka ea hau ka se boleloang ke sephetho sa hau sa liteko.

Haeba sampole ea moroto e na le li-globulin tse ngata kapa tse phahameng ho feta albinine e tloaelehileng, e kanna ea bolela tse latelang:

• Ho ruruha ho matla
• Protheine e sa tloaelehang e aha lisele le litho (amyloidosis)
• Mosebetsi o fokotsehileng oa liphio
• Lefu la liphio ka lebaka la lefu la tsoekere (lefu la tsoekere nephropathy)
• Ho hloleha ha liphio
• Mofuta oa mofets'e oa mali o bitsoang multiple myeloma
• Sehlopha sa matšoao a kenyeletsang protheine morong, liprotheine tse tlase maling, ho ruruha (nephrotic syndrome)
• Tšoaetso e matla ea pampiri ea ho ntša metsi

Ha ho na likotsi ka tlhahlobo ena.

Moroto protheine electrophoresis; PHAHAMISA; Multiple myeloma - UPEP; Waldenström macroglobulinemia - UPEP; Amyloidosis - UPEP

• Tsamaiso ea monna ea ho ntša metsi

CC ea Chernecky, Berger BJ. Liprotheine electrophoresis - moroto. Ka: Chernecky CC, Berger BJ, bahlophisi. Liteko tsa Laboraro le Mekhoa ea Tlhatlhobo. La 6th. St Louis, MO: Elsevier Saunders; 2013: 920-922.

McPherson RA. Liprotheine tse khethehileng. Ka: McPherson RA, Pincus MR, bahlophisi. Henry's Clinical Diagnosis le Tsamaiso ka Mekhoa ea Laboraro. La bo23 la ed. St Louis, MO: Elsevier; 2017: khaolo ea 19.

Rajkumar SV, Dispenzieri A. Multiple myeloma le mathata a amanang le ona. Ka: Niederhuber JE, Armitage JO, Kastan MB, Doroshow JH, Tepper JE, bahlophisi. Abeloff's Clinical Oncology. La 6th. Philadelphia, PA: Elsevier; 2020: khaolo ea 101.