Serum globulin electrophoresis

Teko ea serum globulin electrophoresis e lekanya maemo a liprotheine tse bitsoang globulins karolong ea mokelikeli oa sampole ea mali. Mokelikeli ona o bitsoa serum.

Ho hlokahala sampole ea mali.

Ka laborateng, setsebi se beha sampole ea mali pampiring e khethehileng ebe se sebelisa motlakase. Liprotheine li tsamaea pampiring ebe li theha lihlopha tse bonts'ang palo ea protheine ka 'ngoe.

Latela litaelo tsa hore na u hloka ho itima lijo kapa che pele ho tlhahlobo ena.

Meriana e meng e ka ama litholoana tsa tlhahlobo ena. Mofani oa hao oa tlhokomelo ea bophelo o tla u joetsa haeba ho hlokahala hore u khaotse ho noa meriana. Se ke oa emisa moriana leha e le ofe pele u bua le mofani oa thepa ea hau.

Ha nale e kentsoe ho hula mali, batho ba bang ba utloa bohloko bo itekanetseng. Ba bang ba ikutloa feela ho hlaba kapa ho hlaba. Kamora moo, ho ka ba le ho phatloha kapa ho longoa hanyane. Haufinyane sena sea fela.

Teko ena e etsoa ho sheba liprotheine tsa globulin maling. Ho khetholla mefuta ea li-globulin ho ka thusa ho fumana mathata a itseng a bongaka.

Li-globe li arotsoe ka lihlopha tse tharo: alpha, beta le gamma globulins. Li-globulin tse nang le li-gamma li kenyelletsa mefuta e fapaneng ea lithibela-mafu tse kang immunoglobulins (Ig) M, G le A.

Maloetse a mang a amahanngoa le ho hlahisa li-immunoglobulin tse ngata haholo. Mohlala, Waldenstrom macroglobulinemia ke mofetše oa lisele tse ling tse tšoeu tsa mali. E hokahane le ho hlahisa lithibela-mafu tse ngata haholo tsa IgM.

Mekhahlelo e tloaelehileng ea boleng ke:

• Serum globulin: 2.0 ho isa ho 3.5 gram ka desilitara e ngoe (g / dL) kapa ligrama tse 20 ho isa ho tse 35 ka litara (g / L)
• Karolo ea IgM: li-milligram tse 75 ho isa ho tse 300 ka desilitara (mg / dL) kapa limiligrama tse 750 ho isa ho tse 3 000 ka litara (mg / L)
• Karolo ea IgG: 650 ho 1,850 mg / dL kapa 6.5 ho 18.50 g / L.
• Karolo ea IgA: 90 ho isa ho 350 mg / dL kapa 900 ho isa ho 3,500 mg / L.

Mekhahlelo e tloaelehileng ea boleng e ka fapana hanyane hara lilaboratori tse fapaneng. Li-lab tse ling li sebelisa litekanyo tse fapaneng kapa li leka liteko tse fapaneng. Bua le mofani oa hau ka moelelo oa sephetho sa hau sa liteko.

Liprotheine tse eketsehileng tsa gamma globulin li ka supa:

• Tšoaetso e matla
• Kankere ea mali le masapo a 'mele ho kenyelletsa le myeloma e mengata, le li-lymphomas le leukemias
• Mathata a khaello ea 'mele
• Lefu la ho ruruha la nako e telele (mohlala, ramatiki ea ramatiki le systemic lupus erythematosus)
• Waldenström macroglobulinemia

Ho na le kotsi e nyane haholo e amehang ha ho nkuoa mali a hau. Methapo le methapo li fapana ka boholo ho tloha ho motho e mong ho ea ho o mong, le ho tloha lehlakoreng le leng la 'mele ho ea ho le leng. Ho nka mali ho batho ba bang ho ka ba thata ho feta ho ba bang.

Likotsi tse ling tse amanang le ho huloa mali li nyane, empa li ka kenyelletsa:

• Ho tsoa mali a mangata
• Ho akheha kapa ho ikutloa o le hlooho e boima
• Li-punctures tse ngata ho fumana methapo
• Hematoma (mali a hahiloeng ka tlas'a letlalo)
• Tšoaetso (kotsi e nyane neng kapa neng ha letlalo le robehile)

Li-immunoglobulin tse ngata

• Teko ea mali

CC ea Chernecky, Berger BJ. Immunoelectrophoresis - serum le moroto. Ka: Chernecky CC, Berger BJ, bahlophisi. Liteko tsa Laboraro le Mekhoa ea Tlhatlhobo. La 6th. St Louis, MO: Elsevier Saunders; 2013: 667-692.

Dominiczak MH, Fraser WD. Liprotheine tsa mali le lero la mali. Ka: Baynes JW, Dominiczak MH, bahlophisi. Biochemistry ea Bongaka. La 5th. Philadelphia, PA: Elsevier; 2019: khaolo ea 40.